The t-Phobos Virus was a variant of the t-virus developed by Alex Wesker after the death of Umbrella CEO Oswell E. Spencer. It was named after Phobos, the Greek god of fear.

In his waning years, Spencer ordered Alex to research a method through which he could become immortal, sending Alex to a secret island to conduct the experiments, along with thousands of test subjects. However, after years of supposed experimentation, Alex and her men fled the island, having instead been stealing resources from Spencer and siphoning them into their own projects. Alex then set up a new base on Sushestvovanie Island, where she conducted her own experiment, this time creating a new virus named the t-Phobos Virus.

As late as November 2009, the virus was still only in its prototype stage and undergoing testing on human subjects. By this month, the virus had been engineered to mutate in response to stress. Alex had eleven candidates fitted with sensor bracelets as part of an experiment to measure how long it would take for people to mutate, with three dying immediately and another eight mutating some time after. By January 2010, work on the virus was neared completion and the likelihood of the subject's immediate death was lowered

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